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Application：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：6.25pg/mL
Storage：2-8℃，6 months
Gene ID：18413
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| Name | Mouse Oncostatin M ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | OSM |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 12.5-800pg/mL |
| Sensitivity | 6.25pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P53347 |
| Gene ID | 18413 |
| Background | Oncostatin M (OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays，the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus，proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM. OSM initiates its biological activities by binding to specific cell surface receptors. The gp130，a signal transducing component (beta subunit) of the IL-6，LIF and CNTF receptor complexes，was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R，a gp130-related protein) has now been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R，a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells，as well as on other solid tumor cells，OSM also has growth stimulatory activities on normal fibroblasts，AIDS-Kaposis sarcoma cells，and a human erythroleukemia cell line，TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells，regulation of IL-6 expression in human endothelial cells，and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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