| English Name |
Anti-METTL16 Polyclonal Antibody |
| Chinese Name |
含甲基转移酶域含蛋白10抗体 |
| Host |
Rabbit |
| Clone Type |
Polyclonal Antibody |
| Subtype |
IgG |
| Predicted Reactivity |
Zebrafish;Chicken |
| Dilution Ratio |
WB 1:1000-3000. |
| Target |
METTL16 |
| Synonyms |
2610100D03Rik, 2810013M15Rik, A830095F14Rik, AI846133, Methyltransferase 10 domain containing, Methyltransferase like 16, METT10D, MGC3329, OTTMUSP00000006473, Putative methyltransferase METT10D, RP23-174M12.2. |
| Background |
RNA N6-methyltransferase that methylates adenosine residues at the N6 position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311). |
| Swiss Prot |
Q86W50 |
| Gene ID |
79066 |
| Subcellular Location |
Nucleus Cytoplasm |
| Immunogen |
Recombinant protein of human METTL16 |
| Public Immunogen Range |
1-224aa |
| Calculated MW |
26/64kDa |
| Purification Method |
Affinity purification |
| Storage Buffer |
PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage |
Store at -20℃. Avoid freeze/thaw cycles. |
| Piece |
Piece |
| Specification |
50ul 100ul |
| Reactivity |
Human Monkey Mouse |
| Application |
WB |
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