| Gene Name |
PRKAG1 |
| Synonyms |
5' AMP activated protein kinase gamma 1 subunit;5' AMP activated protein kinase subunit gamma 1;5''-AMP-activated protein kinase subunit gamma-1;AAKG1_HUMAN;AMP activated protein kinase noncatalytic gamma 1 subunit;AMPK gamma 1 chain;AMPK gamma1;AMPK subunit gamma-1;AMPKg;MGC8666;PRKAG 1;PRKAG1;Protein kinase AMP activated gamma 1 non catalytic subunit;protein kinase，AMP-activated，noncatalytic gamma-1 |
| English Name |
Recombinant human PRKAG1 protein |
| Full Name |
protein kinase，AMP-activated，gamma 1 non-catalytic subunit |
| Chinese Name |
5‘-AMP激活蛋白激酶亚基γ-1 |
| Appearance |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
| Purity |
≥85% by SDS-PAGE. |
| Storage |
Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| Transport Condition |
The product is shipped at -20℃ temperature. Upon receipt, store it immediately at the temperature listed below. |
| Concentration |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
P54619 |
| Gene ID |
5571 |
| Reactivity |
Human Mouse Rat (Bovine Pig) |
| Molecular Weight |
35kDa |
| Interval Range |
1-332aa |
| Background |
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Application |
Immunology research |
| Source |
E.coli |
| Purification Method |
NI-NTA affinity purification |
| Solvent |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| Endotoxin |
Not measured |
| Biological Activity |
N0 |
| Lable Information |
With a 6×His tag at the N/C-terminus. |
| Unit |
Piece |
| Specification |
10ug 50ug 100ug 1mg |
English
中文
Manual Download
