| Gene Name |
BCAR3 |
| Synonyms |
BCAR 3;BCAR3;BCAR3_HUMAN;Breast cancer anti estrogen resistance 3;Breast cancer anti estrogen resistance protein 3;Breast cancer anti-estrogen resistance protein 3;Breast cancer antiestrogen resistance 3;dJ1033H22.2;dJ1033H22.2 breast cancer anti estrogen resistance 3;dJ1033H22.2 breast cancer antiestrogen resistance 3;KIAA0554;Novel SH2 containing protein 2;Novel SH2-containing protein 2;NSP 2;NSP2;SH2 containing protein Nsp2;SH2 domain containing protein 3B;SH2 domain-containing protein 3B;SH2D3B |
| English Name |
Recombinant human BCAR3 protein |
| Full Name |
breast cancer anti-estrogen resistance 3 |
| Chinese Name |
乳腺癌抗雌激素抗性蛋白3 |
| Appearance |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
| Purity |
≥85% by SDS-PAGE. |
| Storage |
Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| Transport Condition |
The product is shipped at -20℃ temperature. Upon receipt, store it immediately at the temperature listed below. |
| Concentration |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
O75815 |
| Gene ID |
8412 |
| Reactivity |
Human Mouse Rat (Bovine Monkey) |
| Molecular Weight |
39.4kDa |
| Interval Range |
40-390aa |
| Background |
Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers (PubMed:24216110).Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis (PubMed:24216110).Promotes insulin-mediated membrane ruffling (By similarity).In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1 (PubMed:19086031).Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity (By similarity).May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction (By similarity).Regulates EGF-induced DNA synthesis (PubMed:18722344).Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling (By similarity).Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA (By similarity).Positively regulates integrin-induced tyrosine phosphorylation of BCAR1 (By similarity).Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS (By similarity).However, in a contrasting study, lacks GEF activity towards RAP1 (PubMed:22081014). |
| Application |
Immunology research |
| Source |
E.coli |
| Purification Method |
NI-NTA affinity purification |
| Solvent |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| Endotoxin |
Not measured |
| Biological Activity |
N0 |
| Lable Information |
With a 6×His tag at the N/C-terminus. |
| Unit |
Piece |
| Specification |
10ug 50ug 100ug 1mg |
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