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My CartReactivity：Arabidopsis thaliana
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：830768; 830770
Host：Rabbit
Qty：
Size：
| Name | Anti-ATPB polyclonal Antibody for Plant |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Nicotiana plumbaginifolia;Oryza sativa |
| Dilution Ratio | WB 1:3000 |
| Target | ATPB |
| Background | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Swiss Prot | P83483 |
| Gene ID | 830768; 830770 |
| Subcellular Location | Mitochondrion |
| Immunogen | KLH-conjugated synthetic peptide of ATPB in Arabidopsis thaliana AT5G08670 |
| Calculated MW | 60 kDa |
| Observed MW | 60 kDa |
| Purification Method | Protein A |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. |
| Specification | 50ul 100ul |
| Reactivity | Arabidopsis thaliana |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ATPB antibody diluted at 1:2000;Lane: Arabidopsis thaliana.
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