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Determination of Significance：
Lipid peroxide is produced by the action of oxygen free radicals on unsaturated fatty acid, then resolves to compounds, including malondialdehyde (MDA). The level of lipid peroxidation can be showed by detecting the level of MDA.
Measurement Principle：
Under acidic and high temperature conditions, the brown red 3,5,5- three methyl sulfamethoxazole -2,4-two ketone is synthesized with MDA and thiobarbituric acid (TBA) taking place condensation reaction, and the largest absorption wavelength is 532 nm. After colorimetry, the MDA content in the sample can be estimated.
Self Provided：
Spectrophotometer/microplate reader, water bath, centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
请问MDA GSH SOD的提取液都是一样的么？可以共用么？2023-02-20 11:30:06
Administrator您好，MDA、CAT、SOD、POD这几个常用的盒子提取液是可以通用的，GSH则不是同一系列的了
贴壁细胞收集是用胰酶消化么 可以用细胞裂解液么
2022-11-10 01:32:29
Administrator细胞收集可用胰酶消化，也可细胞刮刀处理。收集的细胞需用等渗缓冲盐溶液洗涤1至2次。
提取细胞上清请参考试剂盒说明书中具体条件，如需用裂解液处理，建议先做预实验，依据具体结果进行调整。
植物材料适用吗？
2022-09-15 17:54:05
Administrator可以检测植物样本，试剂盒没有种属限制。
和BC0020有什么区别2022-08-29 16:43:05
Administrator和BC0020的反应体系大小不一致，BC0020反应体系比较大
蛋白浓度测定可以直接用加入了提取液的上清测定么2022-08-28 20:29:32
Administrator提取液提取的上清可以检测蛋白浓度