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Application：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：117 pg/ml
Storage：2-8℃，6 months
Gene ID：12759
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| Name | Mouse Clusterin ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Clusterin |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 234-15000 pg/ml |
| Sensitivity | 117 pg/ml |
| Specificity | Do not react with Mouse Leptin，TGF-βRI/FcChimera，TGF-βRII/FcChimera etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q06890 |
| Gene ID | 12759 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠Clusterin单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠Clusterin会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠Clusterin抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠Clusterin发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠Clusterin，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠Clusterin浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠Clusterin的浓度。 |
| Background | Clusterin，also known as Apolipoprotein J，Sulfated Glycoprotein 2 (SGP-2)，TRPM-2，and SP-40，is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation，apoptosis，and carcinogenesis (1，2). Clusterin is expressed in adult testis，ovary，adrenal gland，liver，heart，brain，and in many epithelial tissues during embryonic development (3). Mouse Clusterin is synthesized as a precursor that contains two coiled coil domains，two nuclear localization signals (NLS)，and one heparin binding domain (4-6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and? beta chains which are secreted as an approximately 80 kDa N-glycosylated and disulfide-linked heterodimer (7-9). Mature mouse Clusterin shares 77% and 93% amino acid sequence identity with human and rat Clusterin，respectively.? |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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