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My CartApplication：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：78 pg/ml
Storage：2-8℃，6 months
Gene ID：4057
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| Name | Human Lactoferrin/LTF ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | LTF/LF |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 156.25-10000 pg/ml |
| Sensitivity | 78 pg/ml |
| Specificity | Do not react with Human LTF analogue. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P02788 |
| Gene ID | 4057 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人LTF单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人LTF会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人LTF抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人LTF发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人LTF，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人LTF浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人LTF的浓度。 |
| Background | Lactotransferrin，also known as Lactoferrin，Talalactoferrin，and LTF，is a secreted protein that belongs to the transferrin family. Transferrins are iron binding transport proteins that can bind two Fe3+ ions in association with the binding of an anion，usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A，B，and C have opioid antagonist activity. Lactoferrin A shows a preference for mu-receptors，while lactoferricin B and lactoferricin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin / LTF is a globular glycoprotein that is widely represented in various secretory fluids，such as milk，saliva，tears，and nasal secretions. Lactoferrin / LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin / LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration，followed by human milk，then cow milk. Lactoferrin / LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide，fungicide) and is part of the innate defense，mainly at mucose. In particular，lactoferrin provides an antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA，polysaccharides and heparin，and shows some of its biological functions in complexes with these ligands. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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