Cysteine(Cys) Content Assay Kit
Cat.No：BC0185 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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HIF
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> Biochemical Assay Kits > Nitrogen Metabolism Series > Amino Acid Metabolism Series > Cysteine(Cys) Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Cysteine(Cys) Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Protein contains three kinds of sulfur-containing amino acids: methionine, cystine and cysteine(Cys). Cys is the only sulfur-containing amino acid containing sulfhydryl groups, which derived from methionine and could be transformed with cystine. Cys participates in the formation of protein disulfide bonds, which usually is a component of active centers of protein and can provide mercapto groups for other physiological and biochemical reactions. Besides, a large amount of Cys accumulates on skin and mucosal surfaces to maintain elasticity and texture of skin and keep the activity of thiolase in the process of keratin production. It has the functions of whitening, detoxification, inflammation improvement and so on.
Measurement Principle：
The phosphotungstic acid is reduced to tungsten blue by Cys, and the tungsten blue has absorption peak at 600 nm. In this kit, the content of Cys is calculated by measuring the absorbance at 600 nm.
Self Provided：
Refrigerated centrifuge, transferpettor, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, mortar/ homogenizer, phosphoric acid and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Cysteine(Cys) Content Assay Kit
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