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Determination of Significance：
HYP is one of the main components of collagen in the body. Most of the collagen is distributed in the skin, tendon, cartilage and blood vessels et al. Therefore, the content of HYP is an important index reflecting the metabolism and fibrosis degree of collagen tissue.
Measurement Principle：
The sample is hydrolyzed to produce free HYP, which is further oxidized by chloramine T. The oxidized product reacted with p-Dimethylaminobenzaldehyde to produce red compound with characteristic absorption peak at 560 nm. The content of HYP can be calculated by measuring the absorption value of sample hydrolysate at 560 nm.
Self Provided：
Scales, oven, glass tube, centrifuge, water bath, spectrophotometer/microplate reader, micro glass cuvette/96-well plate, 6 mol/L HCl and distilled water.
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