Hydroxyproline(HYP) Content Assay Kit
Cat.No：BC0255 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Nitrogen Metabolism Series > Amino Acid Metabolism Series > Hydroxyproline(HYP) Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Hydroxyproline(HYP) Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
HYP is one of the main components of collagen in the body. Most of the collagen is distributed in the skin, tendon, cartilage and blood vessels et al. Therefore, the content of HYP is an important index reflecting the metabolism and fibrosis degree of collagen tissue.
Measurement Principle：
The sample is hydrolyzed to produce free HYP, which is further oxidized by chloramine T. The oxidized product reacted with p-Dimethylaminobenzaldehyde to produce red compound with characteristic absorption peak at 560 nm. The content of HYP can be calculated by measuring the absorption value of sample hydrolysate at 560 nm.
Self Provided：
Scales, oven, glass tube, centrifuge, water bath, spectrophotometer/microplate reader, micro glass cuvette/96-well plate, 6 mol/L HCl and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Hydroxyproline(HYP) Content Assay Kit
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