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My Cart> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme Ⅱ Series > Isocitrate Dehydrogenase Cytoplasmic(ICDHc) Activity Assay KitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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| Name | Isocitrate Dehydrogenase Cytoplasmic(ICDHc) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
ICDHc widely exist in animals, plants, microorganisms and cultured cells, which catalyzes isocitric acid dehydrogenize and decarboxylate to form α-ketoglutaric acid, reduce NADP+ to form NADPH. ICDHc is a source of NADPH except pentose phosphate pathway in cytoplasm, the enzyme activity will change significantly in adversity.
Measurement Principle：
ICDHc catalyzes NADP+ to form NADPH, the activity of ICDHc can be detected by the increase of NADPH concentration at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, constant temperature water bath, micro quartz cuvette/96 well plate (UV plate), desk centrifuge, adjustable pipette, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Ticlopidine induces embryonic development toxicity and hepatotoxicity in zebrafish by upregulating the oxidative stress signaling pathway
Click to check >>Author：Rong Xu, Pengxiang Xu, Haiyan Wei, Yong Huang, Xiaodan Zhu, Chuanming Lin, Zhimin Yan, Liuyan Xin, Lin Li, Weiming Lv, Shuqin Zeng, Guiyou Tian, Jinze Ma, Bo Cheng, Huiqiang Lu, Yijian Chen
IF：6.8000
Publish_to：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
PMID：37531924
