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Determination of Significance：
ADPG Pyrophosphorylase (AGP) exists mainly in plants, is the main rate-limiting step in plant starch biosynthesis, which catalyzes the reaction of glucose-1-phosphate (G1P) with ATP to produce direct precursor adenosine diphosphate glucose (ADPG) for starch synthesis.
Measurement Principle：
AGP catalyzes the reverse reaction to produce G1P, the added phosphate hexose mutase and 6-phosphate glucose dehydrogenase catalyze the formation of 6-phosphate gluconate and NADPH. In this kit, the activity of AGP is determined by the increase rate of NADPH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/ microplate reader, water bath/incubator, benchtop centrifuge, adjustable pipette, 1mL quartz cuvette, mortar/homogenizer, ice, and distilled water.
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