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Determination of Significance：
PFK (EC 2.7.1.11) is one of the key regulatory enzymes in the process of glycolysis, which widely found in animals, plants, microorganisms and cultured cells. It is responsible for converting fructose-6-phosphate and ATP into fructose-diphosphate and ADP.
Measurement Principle：
PFK catalyzes the formation of fructose-1,6-diphosphate and ADP to from fructose-6-phosphate and ATP. Pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to generate NAD+. The degradation rate of NADH which measured at 340 nm is used to reflect the activity of PFK.
Self Provided：
Spectrophotometer/microplate reader, table centrifuge, water-bath, micro quartz cuvette/96 well flat-bottom UV plate, adjustable pipette, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
