Pyruvate Kinase(PK) Activity Assay Kit
Cat.No:BC0545 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
{{cart_num}}
My Cart>
Biochemical Assay Kits >
Carbohydrate Metabolism Series >
Glycolysis Series >
Pyruvate Kinase(PK) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Pyruvate Kinase(PK) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Pyruvate Kinase (PK, EC 2.7.1.40) is widely exists in animals, plants, microorganisms and cultured cells. It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes in the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of PK activity is of great significance.
Measurement Principle:
PK catalyzes the generation of ATP and pyruvate from phosphoenolpyruvate and ADP. Lactate dehydrogenase further catalyze NADH and pyruvate to generate lactic acid and NAD+. The NADH degradation rate can measured at 340 nm to reflect the activity of PK.
Self Provided:
Spectrophotometer/Microplate reader, table centrifuge, water-bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Validation of protein biological markers of lamb meat quality characteristics based on the different muscle types
Click to check >>Author:Caiyan Huang, Dequan Zhang, Zhenyu Wang, Yingxin Zhao, Christophe Blecker, Shaobo Li, Xiaochun Zheng, Li Chen
IF:8.8000
Publish_to:FOOD CHEMISTRY
PMID:37392625
WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis
Click to check >>Author:Zhang Peng, Fu Hai-Jing, Lv Li-Xia, Liu Chen-Fei, Han Chang, Zhao Xiao-Fan, Wang Jin-Xing
IF:6.5480
Publish_to:Communications Biology
PMID:37012372
FOXM1D Potentiates PKM2-mediated tumor glycolysis and angiogenesis
Click to check >>Author:张玮
IF:6.5000
Publish_to:
PMID:33314660
pH-dependent rearrangement determines the iron-activation and antitumor activity of artemisinins
Click to check >>Author:白光灿
IF:6.1700
Publish_to:
PMID:33359684
Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium
Click to check >>Author:Li Wang, Aike Li, Jun Fang, Yongwei Wang, Lixian Chen, Lin Qiao, Weiwei Wang
IF:5.6000
Publish_to:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID:37511581
BMP4 augments the survival of hepatocellular carcinoma (HCC) cells under hypoxia and hypoglycemia conditions by promoting the glycolysis pathway
Click to check >>Author:钟佳敏
IF:5.1790
Publish_to:
PMID:33791154
FDP-Na-induced enhancement of glycolysis impacts larval growth and development and chitin biosynthesis in fall webworm,Hyphantria cunea (Lepidoptera: Arctiidae)
Click to check >>Author:Shengyu Zhang, Yu Zhang, Haifeng Zou, Xingpeng Li, Hang Zou, Ze Wang, Chuanshan Zou
IF:4.7000
Publish_to:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
PMID:37666596
Novel Umami Peptide IPIPATKT with Dual Dipeptidyl Peptidase-IV and Angiotensin I?Converting Enzyme Inhibitory Activities
Click to check >>Author:陈梦迪
IF:4.1920
Publish_to:
PMID:33949854
Low-protein diets supplemented with glycine improves pig growth performance and meat quality: An untargeted metabolomic analysis
Click to check >>Author:Shengwang Jiang, Wei Quan, Jie Luo, Aihua Lou, Xihong Zhou, Fengna Li, Qingwu W. Shen
IF:3.4710
Publish_to:Frontiers in Veterinary Science
PMID:37143503
KAT2A Promotes the Succinylation of PKM2 to Inhibit its Activity and Accelerate Glycolysis of Gastric Cancer
Click to check >>Author:Zhang Chengpeng, Huang Zonghai
IF:2.6000
Publish_to:MOLECULAR BIOTECHNOLOGY
PMID:37294531