Fatty Acid Synthase(FAS) Activity Assay Kit
Cat.No：BC0555 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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Endocrinology & Hormones
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HIF
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> Biochemical Assay Kits > Fatty Acid Metabolism Series > Fatty Acid Synthase(FAS) Activity Assay Kit
Product Information
| Name | Fatty Acid Synthase(FAS) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, which is one of the key enzymes in sucrose metabolism of higher plants. According to the optimal pH, Ivr can be divided into two types: acid invertase (AI) and neutral invertase (Ni).AI (EC 3.2.1.26) mainly exists in cell vacuole or free space, and the optimal pH is 4.5-5.0 (acid). It can regulate the utilization of sucrose in vacuole and the accumulation of sugar in fruit by degrading sucrose in vacuole.
Measurement Principle：
AI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-dinitrosalicylic acid to form brownish red amino compound, which has a characteristic light absorption at 540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to AI activity.
Self Provided：
Spectrophotometer/microplate reader, desktop centrifuge, water-bath, adjustable pipette, micro glass cuvette/ 96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Integrated physiological, transcriptome and metabolome analyses of the hepatopancreas of the female swimming crab Portunus trituberculatus under ammonia exposure
Click to check >>Author：孟宪亮/刘萍
IF：6.2910
Publish_to：Ecotoxicology and Environmental Safety
PMID：34839137
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