Free fatty Acids(FFA) Content Assay Kit
Cat.No：BC0595 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Fatty Acid Metabolism Series > Free fatty Acids(FFA) Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Free fatty Acids(FFA) Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Free fatty acids (FFA) is the product of fat hydrolysis, also the substrate of fat synthesis. The concentration of FFA in serum is related to lipid metabolism, glucose metabolism and endocrine function.
Measurement Principle：
FFA combines with copper ion to form fatty acid copper salt which is dissolved in chloroform. The content of free fatty acids can be calculated by copper reagent method.
Self Provided：
Spectrophotometer/ Microplate reader, centrifuge, adjustable pipette, micro glass cuvettes/96 well plates, vortex mixer, mortar/homogenizer, one 50mL glass bottle, one 10mL glass bottle, n-heptane, anhydrous Methanol, chloroform, ice, absolute ethanol and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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细胞的脂肪酸含量可以检测么？有没有步骤？ 2022-08-06 17:16:52
Administrator您好，是可以检测的，生化试剂盒没有对样本来源或种属限定，试剂盒技术指标中有检出限和线性范围，取决于提取到的浓度。操作步骤按照500万细胞量对应1mL提取液,破碎方式可以用超声破碎，后面离心条件同组织样本一样，最后计算可以用按蛋白浓度计算的公式。
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