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Dehydroascorbate Reductase(DHAR) Activity Assay Kit

BC0665-241212Manual

Cat.No：BC0665 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Dehydroascorbate Reductase(DHAR) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Dehydroascorbate reductase (DHAR) is an important antioxidant enzyme in plants and a key enzyme that promotes ascorbic acid regeneration in the ascorbate-glutathione oxidation cycle. In the circulation DHAR maintain the normal metabolic level of ascorbic acid in plants through ascorbic acid, and plays an important role in protecting cellular components from oxidative damage.

Measurement Principle：

DHAR catalyzes the reduction of dehydroascorbic acid (DHA) by reducing glutathione (GSH) to produce AsA. GSH can react with 5,5'-dithio-bis- (2-nitrobenzoic acid) (DTNB) to produce 2- Nitro-5-mercaptobenzoic acid (TNB) and glutathione disulfide (GSSG). TNB has maximum light absorption at a wavelength of 412 nm. DHAR activity is calculated by measuring the reduction rate of GSH.

Self Provided：

Low temperature centrifuge, spectrophotometer/microplate reader, water bath, mortar/homogenizer, micro glass cuvette/96 well flat-bottom plate, adjustable pipette, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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