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Glutamate（Glu）Content Assay Kit (WST colorimetry)

BC5215-241212Manual

Cat.No：BC5215 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Glutamate（Glu）Content Assay Kit (WST colorimetry)
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Glu is widely present in animals, plants, microorganisms and cultured cells. It is not only one of the 20 amino acids that make up proteins, but also participates in the synthesis of various amino acids through transamination, and is one of the main sources of amino acids in living organisms. In addition, Glu is also the main active ingredient of monosodium glutamate, and is commonly used in food additives and spice production.

Measurement Principle：

Glutamate dehydrogenase (GDH) catalyzes glutamate and NAD to generate α-ketoglutarate, NADH and NH4+. Under the action of 1-mPMS, WST-1 can react with NADH to produce water-soluble formazan, and calculate glutamate acid content.

Self Provided：

Spectrophotometer / microplate reader, centrifuge, transferpettor, water bath / constant temperature incubator, micro glass cuvettes/96 well plates, mortar/homogenizer, sonicator, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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