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Storage:Store at -20℃，6 months
Determination of Significance：
α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is one of the key enzymes in the regulation of tricarboxylic acid cycle and widely exists in mitochondria of animal, plant, microorganisms and cultured cells, which catalyzes the oxidative decarboxylation of α-ketoglutarate to succinyl coenzyme A.
Measurement Principle：
α-KGDH catalyzes α-ketoglutarate, NAD+ and coenzyme A to form succinyl coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. The activity of α-KGDH is expressed by the formation rate of NADH.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, water-bath/constant temperature incubator, tabletop centrifuge, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
