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Determination of Significance：
Fructose 1,6-bisphosphatase(FBP) also known as fructose-1,6-diphosphatase, which plays a key role in the gluconeogenesis and the synthesis of photosynthetic assimilate sucrose.
Measurement Principle：
FBP catalyzes fructose 1,6-diphosphate and water to produce 6-phosphate fructose and inorganic phosphorus. Glucose-phosphate isomerase and 6-glucose-phosphate dehydrogenase added to the reaction system that catalyze the formation of 6-glucose-phosphate gluconic acid and NADPH in turn. In this kit, the activity of FBP is determined by the increase rate of NADPH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/Microplate Reader, desk centrifuge, adjustable pipette, water bath, micro quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice and distilled water.
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