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NAD Kinase(NADK) Activity Assay Kit

BC1035-241212Manual

Cat.No：BC1035 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	NAD Kinase(NADK) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

NADK (EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyze the phosphorylation of NAD+ to NADP+ in organisms. It can catalyze the phosphorylation reaction of NAD(H) with ATP or inorganic polyphosphate [poly(P)] as a phosphoryl donor to generate NADP(H). Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H).

Measurement Principle：

NADK catalyzes the phosphorylation of NAD+ to produce NADP+, which can be reduced to NADPH by glucose-6-phosphate dehydrogenase. NADPH could reduce oxidized thiazole blue (MTT) through the dehydrogenation of PMS. The absorbance value of MTT at 570 nm can be reflect the activity of NADK.

Self Provided：

Table centrifuge, spectrophotometer/microplate reader, water bath, micro glass cuvette/96 well flat –bottom plate (UV plate), adjustable pipette, mortar/homogenizer, anhydrous ethanol, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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