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Determination of Significance：
Malate Dehydrogenase (MDH, EC 1.1.1.37）widely exist in animal, plant, microbe and cells culture. MDH in mitochondria is one of the key enzymes of TCA cycle, which catalyzes the formation of oxaloacetic acid from malic acid. In contrast, MDH in serum catalyzes the formation of malic acid from oxaloacetic acid. Oxaloacetic acid is an important intermediate that connects several important metabolic pathways. MDH plays an important role in many physiological activities of cells, including mitochondrial energy metabolism, malic acid-aspartic acid shuttle system, reactive oxygen species metabolism and disease resistance. According to the different coenzyme specificity, MDH is divided into NAD- dependent MDH and NADP- dependent MDH. Bacteria usually only contain NAD-MDH. NAD-MDH is distributed in the cytoplasm and mitochondria in eukaryotic cells.
Measurement Principle：
NAD-MDH catalyzes NADH to reduce oxaloacetic acid into malic acid, resulting in the decrease of light absorption at 340nm.
Self Provided：
Spectrophotometer/ Microplate reader, desk centrifuge, water-bath, adjustablepipette, micro quartz cuvette/ 96 well flat-bottom plate (UV plate) and distilled water.
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