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Determination of Significance：
Citrate Synthase (CS,EC 2.3.3.1) is widely exists in animals, plants, microorganism and mitochondrial matrix of cultured cells. It is the first rate-limiting enzyme in the tricarboxylic acid cycle and one of the main regulatory sites.
Measurement Principle：
CS catalyzes acetyl CoA and acetoacetic acid to generate citryl coenzyme A, further hydrolysis to produce citric acid. The reaction promoted the transformation of colorless DTNB to yellow TNB, which has absorption at 412 nm.
Self Provided：
Spectrophotometer/Microplate reader, low temperature centrifuge, water-bath, mortar/homogenizer,adjustablepipette, micro glass cuvette / 96 well flat-bottom plate and distilled water.
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