Alcohol Dehydrogenase(ADH) Activity Assay Kit
Cat.No:BC1085 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Alcohol Dehydrogenase(ADH) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Alcohol Dehydrogenase(ADH) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Alcohol dehydrogenase (ADH) is a key enzyme in the metabolism of short chain alcohols. It catalyzes the reversible conversion of ethanol and acetaldehyde, and plays an important role in many physiological processes. In mammals, ADH is mainly produced in the liver. Liver damage causes ADH to be released into serum. The activity of serum ADH reflects whether the liver function is abnormal.
Measurement Principle:
ADH catalyzes the reduction of acetaldehyde by NADH to ethanol and NAD+. NADH has an absorption peak at 340 nm but NAD+ not, the activity of ADH is calculated by measuring the rate of absorbance decline at 340 nm.
Self Provided:
Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice, Low Temperature Centrifuge, Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/96 Well Flat-bottom Plate (UV plate), Water Bath; Adjustable Pipette and Distilled Water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Apple Consumption Protects against Acute Ethanol-Induced Liver Injury in Rats
Click to check >>Author:Chen Wang, Chang-Ning Ma, Xiao-Long Liu, Quan Sun, Qian Zhang, Ying-Ying Lin, Cheng-Yu Yan, Da-Gang Hu
IF:2.8380
Publish_to:Applied Sciences-Basel
PMID: