Creatine Kinase(CK) Activity Assay Kit
Cat.No:BC1145 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Creatine Kinase(CK) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Name | Creatine Kinase(CK) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Creatine kinase (CK) (EC 2.7.3.2) is also known as creatine phosphokinase, which mainly exists in heart, muscle and brain. It can reversibly catalyze the trans-phosphoryl reaction between creatine and ATP. It is an important kinase directly related to cell energy transport, muscle contraction and ATP regeneration.
Measurement Principle:
CK catalyzes creatine phosphate and ADP to generate creatine and ATP, hexokinase catalyzes ATP and glucose to generate glucose-6-phosphate, and glucose-6-phosphate dehydrogenase catalyzes glucose-6-phosphate and NADP+ to generate NADPH, resulting in an increase of 340 nm light absorption value, which is used to express CK enzyme activity.
Self Provided:
Scales, low temperature centrifuge, constant temperature water bath, spectrophotometer/microplate reader, micro quartz cuvette/96 wellflat-bottom(UV) plate and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Click to check >>Author:Song Liu, Jianmin Ren, Shiyi Liu, Xiaoyan Zhao, Huiru Liu, Tingting Zhou, Xueying Wang, Haoyue Liu, Lei Tang, Heping Chen
IF:5.1950
Publish_to:EUROPEAN JOURNAL OF PHARMACOLOGY
PMID:37149277