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Determination of Significance：
TrxR is a NADPH-dependent dimer selenase and includes FAD structure domain. TrxR belongs to pyridine nucleotide-disulfide REDOX enzyme, and form thioredoxin system with thioredoxin and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduce to GSH, which is the key enzyme in glutathione REDOX cycle.
Measurement Principle：
TrxR catalyzes NADPH to reduceDTNB form TNB and NADP+, TNB has a absorbance at 412 nm, but reduced glutathione reacts with DTNB to form TNB, so the 2-Vinylpyridine in this kit can inhibit reduced glutathione in sample, the activity of TrxR can be calculated by detecting increase rate of TNB at 412 nm.
Self Provided：
Spectrophotometer/microplate reader, ultra-micro glass cuvette/96 well flat-bottom plate,water bath, low temperature centrifuge, mortar/homogenizer, adjustable pipette,distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
用bca蛋白检测试剂盒测蛋白浓度可以吗？还是用其他的2022-07-27 10:07:51
Administrator您好，建议用Bradford法检测，但提取液中本身含有一定蛋白浓度，注意减去提取液本身蛋白浓度