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Determination of Significance：
Glutathione is a natural tripeptide composed of glutamic acid (Glu), cysteine (Cys) and glycine (Gly). It is a kind of compound containing sulfhydryl group (-SH), which widely exists in animal tissue, plant tissue, microorganism and yeast.
Measurement Principle：
Glutathione can react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to produce 2-nitro-5-mercaptobenzoic acid and glutathione disulfide (GSSG). 2-nitro-5-mercaptobenzoic acid is a yellow product with the maximum absorption at 412 nm.
Self Provided：
Analytical balance, mortar/homogenizer, low temperature centrifuge, water bath, adjustable pipette, spectrophotometer/microplate reader, micro glass cuvette or 96 well flat-bottom plate and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
您好，说明书中加样那一步，标准溶液的是什么浓度2023-07-17 09:59:36
Administrator您好，试剂盒需要根据说明书中标准溶液配制，配制不同浓度标准品做一条标准曲线
说明书里细胞处理的反复冻融2-3次（可在液氮中冻结，37摄氏度水浴中溶解）的处理，可以改为在-80摄氏度的冰箱里冻结，在37摄氏度溶解吗？冻结时间和溶解时间都要求多长啊2023-06-02 17:30:39
Administrator您好，可以使用-80摄氏度冰箱冻结，冻结时间和溶解时间没有特别要求，反复冻融后细胞能完全破碎就可以。
这个试剂盒测出来的就是还原型GSH的量了是吗？不需要测总GSH和GSSG？2023-05-23 10:22:20
Administrator您好，这个试剂盒的是样本中还原型谷胱甘肽的含量，不包含氧化型谷胱甘肽含量。
这个试剂盒是适用于所有种属吗？2022-09-07 20:49:44
Administrator生化试剂盒没有种属限制