Oxidized Glutathione(GSSG) Content Assay Kit
Cat.No：BC1185 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Glutathione Series > Oxidized Glutathione(GSSG) Content Assay Kit
Product Information
| Name | Oxidized Glutathione(GSSG) Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Oxidized Glutathione(GSSG) is an oxidized form of glutathione (GSH), also known as dithioneglutathione, formed by the oxidation of two molecules of glutathione. GSSG is reduced to GSH by glutathione reductase, so most of the body is in the reduced form. The determination of GSH and GSSG content and ratio of GSH/GSSG in cells can reflect the redox status of cells. This kit utilizes reaction of glutathione and 5, 5'-dithiobis-2-nitrobenoic acid (DTNB) to produce 5-thio-2- nitrobenzoic acid.
Measurement Principle：
5-thio-2-nitrobenzoic acid has the largest absorption at wavelength of 412nm, and 2-Vinylpyridine inhibit reduced glutathione in the original of samples, and then using glutathione reductase to reduce GSSG to GSH, determining the content of Oxidized Glutathione.
Self Provided：
Analytical balance, mortar/homogenizer, refrigerated centrifuge, water-bath, adjustable pipette, spectrophotometer/ microplate reader, micro glass cuvette/96 well flat-bottom plate.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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