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Determination of Significance：
Oxidized Glutathione(GSSG) is an oxidized form of glutathione (GSH), also known as dithioneglutathione, formed by the oxidation of two molecules of glutathione. GSSG is reduced to GSH by glutathione reductase, so most of the body is in the reduced form. The determination of GSH and GSSG content and ratio of GSH/GSSG in cells can reflect the redox status of cells. This kit utilizes reaction of glutathione and 5, 5'-dithiobis-2-nitrobenoic acid (DTNB) to produce 5-thio-2- nitrobenzoic acid.
Measurement Principle：
5-thio-2-nitrobenzoic acid has the largest absorption at wavelength of 412nm, and 2-Vinylpyridine inhibit reduced glutathione in the original of samples, and then using glutathione reductase to reduce GSSG to GSH, determining the content of Oxidized Glutathione.
Self Provided：
Analytical balance, mortar/homogenizer, refrigerated centrifuge, water-bath, adjustable pipette, spectrophotometer/ microplate reader, micro glass cuvette/96 well flat-bottom plate.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
