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Determination of Significance：
GCL (Glutamylcysteineligase) is the rate limiting enzyme of GSH synthesis. GSH has feedback inhibition to GCL. GCL gene express manipulated by many factors, such as oxidants, antioxidants, growth factors and inflammatory factors. The activity of GCL has important influence to GSH content and ratio of GSH/GSSG.
Measurement Principle：
In the presence of ATP and Mg2+, GCL catalyzes the synthesis of γ-glutamyl cysteine from glutamate and cysteine, and Dephosphorylation of ATP produce inorganic phosphorus molecules. The activity of GCL can be calculated by measuring the increasing rate of inorganic phosphorus.
Self Provided：
Spectrophotometer/Microplate reader, Refrigerated centrifuge, water bath, adjustablepipette, micro glass cuvette/96 well flat-bottomplate, concentrated sulfuric acid and distilled water.
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