Determination of Significance:
AsA is also called Vitamin C.AsA is the substrate of coenzyme, free radical scavenger, electron copolymer/receptor, biosynthesis of oxalate and tartrate. As the most important antioxidant in plant cells,AsA has important function in protecting chloroplast from oxidizing. It is also one of the important indexes to measure the quality of crop products.
Measurement Principle:
Ascorbate oxidase (AAO) catalyze AsA to form DHA. According to detect the oxidize rate of AsA, can calculate the content of AsA.
Self Provided:
Mortar, ice, low temperature centrifuge, ultravioletspectrophotometer/microplate reader, micro quartz cuvette/96 well flat-bottom plate (UV plate), adjustable pipette and distilled water.
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