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Determination of Significance：
Nitrite reductase (NiR) is a key enzyme in the reduction of nitrite nitrogen, and plays an important role in the biogeochemical?nitrogen cycle, which is widely found in microbes and plants. It catalyze the reduction of nitrite nitrogen, reduce the accumulation of nitrite nitrogen and its toxic effect on the growth and development of organisms.
Measurement Principle：
Nitrite reductase can reduce NO2- to NO, and reduce the NO2- in the sample to participate in the diazotization reaction to produce a purple-red compound, that is, the change in absorbance at 540nm can reflect the activity of nitrite reductase in soil.
Self Provided：
Spectrophotometer/Microplate reader, adjustable transferpettor, balance, mortar/homogenizer, centrifuge, micro glass cuvette/ 96-well flat-bottom plate, ice and distilled water.
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