English
中文
Determination of Significance：
GPT is widely found in animals, plants, microbes and cultured cells, which is an important enzyme in amino acid metabolism. It catalyzes the transamination of amino acid and keto acid. In addition, GPT activity is very high in mammalian liver cells. GPT is released into the blood when liver cells necrotic, serum GPT activity is significantly increased. Therefore, GPT is recommended as the most sensitive indicator of liver damage by the World Health Organization.
Measurement Principle：
GPT catalyzes the transamination reaction of alanine and α-ketoglutarate to generate pyruvate and glutamic acid; the addition of 2,4-dinitrophenylhydrazine solution not only terminates the above reaction, but also increases Into phenylpyrene pyruvate; which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.
Self Provided：
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, water bath, desk centrifuge, transferpettor, distilled water, ice and mortar/homogenizer.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
这个试剂盒是检测人的谷丙转氨酶，还是小鼠的？2023-03-06 11:44:37
Administrator您好，这个试剂盒没有物种特异性，人，动物，植物组织和细胞样本都可以检测。
BC1555在检测时使用的96孔板可否用细胞培养96孔板？2022-09-27 17:04:24
Administrator如果用酶标仪检测使用96孔酶标板而不是细胞培养板，或者用分光光度计配合微量比色皿去测。