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Determination of Significance：
GOT is widely found in animals, plants, microbes and cultured cells. It catalyzes the reversal of amino reactions and is an important enzyme in amino acid metabolism. In addition, GOT is the highest in cardiomyocytes and is commonly used as an assisted examination of myocardial infarction and myocarditis in clinical practice. The serum concentration of liver damage can also be increased.
Measurement Principle：
GOT catalyze α-ketoglutaric acid react with aspartate to produce glutamic acid and oxaloacetic acid. Oxaloacetic acid is further decarboxylated to form pyruvate, pyruvate can react with 2, 4-dinitrophenylhydrazine to produce 2,4-dinitrophenylhydrazone, which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.
Self Provided：
Spectrophotometer/microplate reader, water bath, desk centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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