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Determination of Significance：
Animal liver and kidney are the main organs of amino acid metabolism, so the changes of amino acids in urine can reflect the physiological state of liver and kidney. In addition, amino acids can also respond to burns, typhoid and other aspects of the situation. The content of amino acids in plants have a great significance to the study of nitrogen’s metabolism, absorption, transport, assimilation and nutritional status under different conditions and at different stages of growth and development.
Measurement Principle：
α-Amino of amino acid can react with hydrated ninhydrin to produce blue purple compound, which has absorption peak at 570 nm, and the content of amino acid is calculated by measuring absorbance of 570 nm.
Self Provided：
Desk centrifuge, spectrophotometer/microplate reader, water bath, micro glass cuvette/96 well flat-bottom plate, transferpettor, mortar/homogenizer, absolute ethyl alcohol, ice and distilled water.
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请问用过一次之后的保存时间是多少2022-07-08 12:55:54
Administrator建议一个月内使用完