Glutamic Acid(Glu) Content Assay Kit
Cat.No:BC1585 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Glutamic Acid(Glu) Content Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Glutamic Acid(Glu) Content Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
lu is widely found in animals, plants, microbes and cultured cells. It’s not only one of the 20 amino acids that makes up the protein, but also participates in the synthesis of many kinds of amino acids by transaminination It is one of the main amino sources in organism. Besides, Glu is also the main active ingredient of monosodium glutamate, often used as food additive and spice production.
Measurement Principle:
GDH catalyze glutamic acid and NAD to form α-ketoglutaric acid, NADH and NH4+, the absorbance of 340 nm was increased and the content of glutamate was calculated by measuring the absorbance of 340 nm.
Self Provided:
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, transferpettor, water bath, micro quartz cuvette/96 well plate (UV plate), mortar/homogenizer, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Publish_to:iScience
PMID:37408687