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Determination of Significance：
Starch branching enzyme(SBE) exists mainly in plants, is a key enzyme of amylopectin biosynthesis. The determination of SBE activity is of great significance in the study of starch biosynthesis, selection of high-quality crop varieties and genetic improvement of quality.
Measurement Principle：
The complex formed by the combination of starch and iodine has a characteristic absorption peak at 660nm. SBE can cut off the side branches of amylopectin, thus reducing the absorption value of starch iodine complex at 660 nm. In a certain time, the percentage of absorbance decrease can reflect SBE activity.
Self Provided：
Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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