Your Position: Home > Biochemical Assay Kits > Other Biochemical Series > Soil Series > Soil Chitinase Activity Assay Kit

Soil Chitinase Activity Assay Kit

BC1935-241212Manual

Cat.No：BC1935 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at 2-8℃，6 months

Qty：

Size：

Add to cart

Product Information Experimental Images Related Products Related Paper Technical Q&A

Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Soil Chitinase Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Chitin is the second largest class of biopolymers in nature, which is second only to cellulose. Due to its slow decomposition and large accumulation, it is easy to cause serious environmental pollution. Chitinase is an important enzyme affecting nitrogen mineralization in soil, and its decomposition of chitin controls the key step of nitrogen cycle.

Measurement Principle：

Chitinase hydrolyzes chitin to produce N-acetylglucosamine. The intermediate compound produced by the reaction of N-acetylglucosamine and alkali can further react with p-Dimethylaminobenzaldehyde to produce a chromogenic substance. The chromogenic substance has a characteristic absorption peak at 585 nm. The increasing rate of absorbance reflects the activity of chitinase.

Self Provided：

Spectrophotometer/microplate reader, Low tempareture centrifuge, transferpettor, oscillator, micro glass cuvette/96 well flat-bottom plate, mortar, 30-50 mesh sieve (or smaller), distilled water and toluene, water bath or constant temperature incubator.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

Experimental Images

Technical Q&A

Q&A （Total 0 User Q&A）

There is not question and answer now, come and send an inquiry please.

My question

Rank：

My question：

Identifying code：
captcha