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Determination of Significance：
Soil laccase (SL) is a polyphenol oxidase containing copper. It belongs to the ceruloplasmic oxidase family. It is widely distributed in fungi and higher plants. At the same time, it is widely used in pulp biobleaching, environmental pollutant degradation, lignocellulose degradation and biological detection.
Measurement Principle：
Laccase can decompose substrate ABTS to produce ABTS free radicals. Its absorption coefficient at 420nm is much higher than that of ABTS. Laccase activity can be calculated by measuring the increasing rate of ABTS radicals.
Self Provided：
Balance, low temperature desk centrifuge, constant temperature foster box/water-bath, transferpettor, spectrophotometer/microplate reader, oscillating instrument, micro glass cuvette/96 well flat-bottom plate, mortar, 30-50 mesh sieve.
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