Isocitrate Lyase(ICL) Activity Assay Kit
Cat.No:BC2035 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
{{cart_num}}
My Cart>
Biochemical Assay Kits >
Other Biochemical Series >
Other Series >
Isocitrate Lyase(ICL) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Isocitrate Lyase(ICL) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
ICL (ec4.1.3.1) mainly exists in plants and microorganisms. During the germination process of oil seeds, fat is converted into carbohydrate through glyoxylate cycle and other processes. ICL is one of the key enzymes in glyoxylate cycle.
Measurement Principle:
The degradation of isocitric acid to glyoxylic acid and succinic acid is catalyzed by ICL. Glyoxylic acid and NADH generate ethanol and NAD under the action of LDH. NADH has a characteristic absorption peak at 340nm. Monitoring the reduction rate of 340nm absorbance can indirectly reflect the activity of ICL.
Self Provided:
Spectrophotometer/ Microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro quartz cuvette/ 96 well flat-bottom plate, mortar / homogenizer, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Cr(VI) Reduction and Fe(II) Regeneration by Penicillium oxalicum SL2-Enhanced Nanoscale Zero-Valent Iron
Click to check >>Author:Yating Luo, Jingli Pang, Cheng Peng, Jien Ye, Bibo Long, Jianhao Tong, Jiyan Shi
IF:11.4000
Publish_to:ENVIRONMENTAL SCIENCE & TECHNOLOGY
PMID:37474249
Single-Component Self-Healing Antibacterial Anti-Inflammatory Intracellular-Antioxidative Poly(itaconic acid-pluronic) Hydrogel for Rapid Repair of MRSA-Impaired Wound
Click to check >>Author:Junping Ma, Ting Li, Meng Luo, Bo Lei
IF:9.5000
Publish_to:ACS Applied Materials & Interfaces
PMID:37394732