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Determination of Significance：
Hydrogen sulfide (H2S) is a new type of gaseous signal molecule. It is a neurotransmitter that exists in the brain. The physiological concentration of H2S has an important regulatory effect on the long-term enhancement of the hippocampus of the nervous system. It also plays an important pathophysiological effect on the process of spontaneous hypertension, hemorrhagic shock and liver cirrhosis.
Measurement Principle：
H2S can react with N, N-dimethyl-p-phenylenediamine and ferric ammonium sulfate to form methylene blue. Methylene blue has a maximum absorption peak at 665nm. The H2S content can be calculated by measuring the absorbance value.
Self Provided：
Spectrophotometer/microplate reader, desk centrifuge, pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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