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Determination of Significance：
Vitamin B6 (VB6), also known as pyridoxine, which includes pyridoxine, pyridoxal and pyridoxamine, is present in the form of phosphates in the body. VB6 is a water-soluble vitamin, which is plentiful in meat, whole grains, vegetables and nuts. VB6 participate in the metabolism of a variety of proteins and amino acids in the body, which has an extremely important role in the organism.
Measurement Principle：
Under the action of strong oxidant, VB6 and 4-aminoantipyrine form a stable yellow compound which has characteristic absorption peak at 400 nm. In this kit, the content of VB6 is calculated by measuring the absorbance at 400 nm.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, transferpettor, water bath, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, EP tube, ice and distilled water.
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