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Storage:Store at -20℃，6 months
Determination of Significance：
Phosphoenolpyruvate carboxylase (EC 4.1.1.31) is widely found in plants and microorganisms. It catalyzes the irreversible reaction of phosphoenolpyruvate and carbon dioxide to oxaloacetic acid. It is also a key enzyme for C4 plants and cam plants to fix CO2. It plays an important role in regulating the operation of tricarboxylic acid cycle.
Measurement Principle：
PEPC can catalyze phosphoenolpyruvate and carbon dioxide to form oxaloacetate and HPO42-. Malate dehydrogenase can catalyze oxaloacetate and NADH to produce malate and NAD+. The rate of NADH reduction was measured at 340 nm and PEPC activity was calculated.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well UV plate, pipette, mortar/homogenizer, ice and distilled water.
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