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Fructose-1，6-diphosphate(FDP)Content Assay Kit

BC2245-241212Manual

Cat.No：BC2245 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Fructose-1，6-diphosphate(FDP)Content Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/96S

Measurement significance:

Fructose-1,6-Diphosphate (FDP) is an important intermediate product in the process of glycolysis.It has a regulatory effect on a variety of enzymes,and can improve cell energy metabolism,increase energy utilization anti-arrhythmia and anti-tissue peroxidation.It is widely used in clinical medicine.

Determination method:

Aldolase catalyzes fructose 1,6-diphosphate to produce glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.Under the action of triose phosphate isomerase and α-glycerophosphate dehydrogenase,NADH and dihydroxyacetone phosphate are catalyzed to produce NAD+ and α-glycerophosphate, causing a decrease in absorbance at 340 nm.The content of fructose 1,6-diphosphate can be calculated by measuring the change of NADH.

Instruments and supplies to be provided:

Spectrophotometer/Microplate Reader,balance,low temperature desktop centrifuge,water bath/thermostatic incubator,1mL quartz cuvette,adjustable pipette,mortar/homogenizer / cell ultrasonic disruptor,vortex oscillator,ice,distilled water and EP tube.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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