Determination of Significance:
Fructose is one kind of ketohexose and an isomeride of glucose, which exists widely in the pμlp of fruits and honey with free state. It can be combined with glucose to form sucrose. Fructose is the sweetest monosaccharide and is used widely in the production of food, medicine and health care products.
Measurement Principle:
In acidic conditions, fructose reacts with resorcinol to form colored substances which has characteristic absorption peaks at 480 nm.
Self Provided:
Spectrophotometer/ microplate reader, water bath, centrifuge, adjustable transferpettor, micro glass cuvette/ 96 well flat-bottom plate, mortar/homogenizer, distilled water.
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