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Determination of Significance：
Polygalacturonase (PG) is a type of pectinase that is widely found in plants, bacteria and fungi. It catalyzes the decomposition of polygalacturonic acid, and plays an important role in softening fruits, pollen pollination, maturity of seeds, and shedding of organs. When pathogenic bacteria infect host plants, they can secrete polygalacturonase to degrade the host the cell wall, in turn, leads to the development of the disease course.
Measurement Principle：
PG hydrolyzes polygalacturonic acid to generate galacturonic acid, and galacturonic acid reacts with DNS reagents to produce a brown-red substance with a characteristic absorption peak at 540 nm. Pectinase activity can be calculated by measuring the change in absorbance at 540 nm.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, water bath, mortar/ homogenizer/cell ultrasonic crusher, micro glass cuvette/ 96 well flat-bottom plate, transferpettor, ice and distilled water.
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