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Determination of Significance：
Cytochrome P450 enzymes are a group of isoenzymes mainly found in the liver. They play an important role in the metabolism of exogenous substances, especially the metabolism of drugs and poisons. As an important member of the P450 enzyme system, NCR catalyzes the reduction and regeneration of oxidized P450.
Measurement Principle：
NCR catalyzes the reduction of oxidized cytochrome C by NADPH to produce reduced cytochrome C, which has a characteristic absorption peak at 550nm. NCR activity is calculated by measuring the rate of increase in absorbance at 550nm.
Self Provided：
Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, desk centrifuge, transferpettor, micro glass cuvette/96-well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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