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Serum Total Iron Binding Capacity(TIBC) Assay Kit

BC2865-241212Manual

Cat.No：BC2865 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at 2-8℃，6 months

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Product Information

Name	Serum Total Iron Binding Capacity(TIBC) Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	100T/96S

Determination of Significance：

Total iron-binding capacity (TIBC) refers to the ability of serum transferrin to bind iron, and its content is closely related to the diseases such as iron deficiency anemia and acute hepatitis.

Measurement Principle：

Fe²⁺ reacts with ferrozine to form a fuchsia compound which has an absorption peak at 562nm. In alkaline condition, serum transferrin can bind with Fe³⁺, and the remaining unbound Fe³⁺ can be reduced to Fe²⁺. So the absorbance A1 is positively correlated with Fe³⁺. After acidification, the transferrin-bound Fe³⁺ is released and further reduced to Fe²⁺. The absorbance A2 has a positive correlation with Fe³⁺, A2 minus A1 was proportional to TIBC.

Self Provided：

Spectrophotometer/microplate reader, water bath/ constant temperature foster box, centrifuge, micro glass cuvette/ 96 well plate, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.