Determination of Significance:
Soil hydrargyrum pollution can be transmitted and enriched through the food chain, posing a threat to plant, animal, and human health. Mine development, industrial processing, agricultural production, and domestic waste often cause soil mercury pollution, so evaluating and preventing soil heavy metal pollution often requires measuring soil mercury content.
Measurement Principle:
After the soil digested, hydrargyrum exists in the form of Hg2+; Hg2+ can form an orange complex with dithizone, and after dissolving in chloroform, measuring the absorbance at 490nm, the S-Hg content can be calculated.
Self Provided:
Spectrophotometer/ microplate reader, water bath, centrifuge, micro glass cuvette/ 96-well plate, adjustable pipette, 50 mesh sieve (can be smaller), concentrated sulfuric acid (H2SO4), concentrated nitric acid (HNO3), chloroform (CCl3) and distilled water.
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