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Determination of Significance：
Soil nitrite reductase (S-NiR) is one of the key enzymes in denitrification. It is a reductase produced by soil denitrifying bacteria. It can reduce NO2- to NO. The activity reflects the conversion efficiency of nitrogen in the process of biodegradation, and provides a certain basis for the study of nitrogen conversion.
Measurement Principle：
Nitrite reductase can reduce NO2- to NO, and reduce the NO2- in the sample to participate in the diazotization reaction to produce a purple-red compound, that is, the change in absorbance at 540nm can reflect the activity of nitrite reductase in soil.
Self Provided：
Spectrophotometer/ Microplate reader, low temperature desk centrifuge, water-bath, adjustable transferpettor, mortar, micro glass cuvette/ 96-well flat-bottom plate, sieve (30-50 mesh, or smaller), ice and distilled water.
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