Soil Hydroxylamine Reductase(S-HR)Activity Assay Kit
Cat.No:BC3015 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
Qty:
Size:
{{cart_num}}
My Cart>
Biochemical Assay Kits >
Other Biochemical Series >
Soil Series >
Soil Hydroxylamine Reductase(S-HR)Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
Qty:
Size:
Name | Soil Hydroxylamine Reductase(S-HR)Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Unit | Kit |
Specification | 100T/48S |
Determination of Significance:
Soil hydroxylamine reductase can reduce the intermediate product hydroxylamine formed in the process of soil nitrogen metabolism to ammonia, and the reduced compounds in the soil can be used as hydrogen donors. Its strength affects the ammonia volatilization loss of nitrogen in the process of soil nitrogen metabolism, and indirectly affects the utilization efficiency of nitrogen.
Measurement Principle:
Fe3+ in ammonium ferric sulfate can oxidize hydroxylamine to nitrogen and reduce itself to Fe2+, Fe2+ forms orange red complex with o-phenanthroline under weak acid condition, orange red complex has absorption peak at 510nm. hydroxylamine reductase acts on hydroxylamine that could reduce the amount of formation of complex, and the decrease of absorption value at 510nm can reflect the activity of hydroxylamine reductase.
Self Provided:
Spectrophotometer/ Microplate reader, scales, centrifuge, transferpettor, micro glass cuvette/ 96-well flat-bottom plate, vortex shaker, nitrogen blower, mortar, 30-50 mesh sieve and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Trade-offs between wheat soil N2O emissions and C sequestration under straw return,elevated CO2 concentration,and elevated temperature
Click to check >>Author:Jing Yang, Guojun Liu, Haiyan Tian, Xin Liu, Xingyu Hao, Yuzheng Zong, Dongsheng Zhang, Xinrui Shi, Aiping Wang, Ping Li, Shu Kee Lam
IF:10.7530
Publish_to:SCIENCE OF THE TOTAL ENVIRONMENT
PMID:37247731